Journal: PLoS Pathogens

Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

doi: 10.1371/journal.ppat.1002575

Figure Lengend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

Techniques: Injection, Immunohistofluorescence, Expressing, Infection

Journal: PLoS Pathogens

Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

doi: 10.1371/journal.ppat.1002575

Figure Lengend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

Techniques: Injection, Immunohistofluorescence, Immunofluorescence, Expressing

Journal: PLoS Pathogens

Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

doi: 10.1371/journal.ppat.1002575

Figure Lengend Snippet: Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

Techniques: Immunohistofluorescence, Expressing, Infection, Injection

Journal: PLoS Pathogens

Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

doi: 10.1371/journal.ppat.1002575

Figure Lengend Snippet: Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

Techniques: Injection, Immunohistofluorescence, Expressing, Infection

Journal: The Journal of Cell Biology

Article Title: A Novel 14-Kilodalton Protein Interacts with the Mitogen-Activated Protein Kinase Scaffold Mp1 on a Late Endosomal/Lysosomal Compartment

doi:

Figure Lengend Snippet: Interaction between p14 and MP1 in vivo II: colocalization and comislocalization. HeLa cells were transiently cotransfected with X-p14 (top and middle panel) or X-p14–CAAX (bottom panel) and MP1-myc 6 . After 24 h incubation, the cells were fixed and processed for indirect immunofluorescence using anti–LAMP-1, anti-Xpress and anti-myc antibodies. Bars, 10 μm.

Article Snippet: Confocal images were obtained with a Leica TCS NT confocal microscope and processed using the Imaris and colocalization software packages (Bitplane AG) after deconvolution using measured point-spread functions with the Huygens software (Scientific Volume Imaging).

Techniques: In Vivo, Incubation, Immunofluorescence